HeadHodge
Bula To Eternity
Now that spectrometers are relatively inexpensive and more easily obtainable by all, I've been investigating a way to use one to test kava as a cheap and easy alternative to HPLC equipment and techniques.
My knowledge on this topic is very limited and I've more and less run into a wall blocking my progress. So I'm posting this in hopes that there are others out there that can help break down the walls of my ignorance.
I've included two papers that have been written showing the feasibility of doing this, but they both lack the details needed for me to act on the concepts.
Based on my interpretation of both papers, they both use pretty much similar techniques to use reflective spectroscopy to analyse kava samples with results closely resembling those of HPLC testing.
It appears that the way they do it is to do spectral tests on a sample of kava. The result is an unintelligible spectral graph over a wide spectrum of wavelengths. In order to decode this graph they took the exact same kave and performed HPLC tests on it to get specific information that can be easily interpreted. They then use statistical analysis with this data to essentially map the HPLC to spectrometer test results to create a decoder ring that can be used to interpret the results of the spectral graphs obtained from the spectrometer tests.
They use reflective spectroscopy to analyse dry samples of fine kava powder, with the thinking that this would be the best way to do on site testing in Vanuatu.
But I would like to use absorptive spectroscopy by extracting kava in a solvent instead because the equipment needed is much cheaper.
What I really need right now is for someone to help me figure out how to recreate the techniques that the papers use to map the resulting spectral graphs to their equivalent HPLC test results.
Regards
My knowledge on this topic is very limited and I've more and less run into a wall blocking my progress. So I'm posting this in hopes that there are others out there that can help break down the walls of my ignorance.
I've included two papers that have been written showing the feasibility of doing this, but they both lack the details needed for me to act on the concepts.
Based on my interpretation of both papers, they both use pretty much similar techniques to use reflective spectroscopy to analyse kava samples with results closely resembling those of HPLC testing.
It appears that the way they do it is to do spectral tests on a sample of kava. The result is an unintelligible spectral graph over a wide spectrum of wavelengths. In order to decode this graph they took the exact same kave and performed HPLC tests on it to get specific information that can be easily interpreted. They then use statistical analysis with this data to essentially map the HPLC to spectrometer test results to create a decoder ring that can be used to interpret the results of the spectral graphs obtained from the spectrometer tests.
They use reflective spectroscopy to analyse dry samples of fine kava powder, with the thinking that this would be the best way to do on site testing in Vanuatu.
But I would like to use absorptive spectroscopy by extracting kava in a solvent instead because the equipment needed is much cheaper.
What I really need right now is for someone to help me figure out how to recreate the techniques that the papers use to map the resulting spectral graphs to their equivalent HPLC test results.
Regards
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